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Cofilin antibody (Tyr88)

This Rabbit Polyclonal antibody specifically detects Cofilin in WB, IF and IHC (p). It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN319381

Quick Overview for Cofilin antibody (Tyr88) (ABIN319381)

Target

See all Cofilin (CFL1) Antibodies
Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity

  • 145
  • 94
  • 92
  • 7
  • 5
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
Human, Mouse

Host

  • 141
  • 21
  • 2
  • 1
Rabbit

Clonality

  • 141
  • 23
Polyclonal

Conjugate

  • 87
  • 10
  • 10
  • 7
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Cofilin antibody is un-conjugated

Application

  • 87
  • 64
  • 61
  • 44
  • 41
  • 41
  • 36
  • 33
  • 23
  • 22
  • 13
  • 7
  • 3
  • 3
  • 3
  • 2
  • 2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Binding Specificity

    • 18
    • 17
    • 16
    • 15
    • 11
    • 9
    • 8
    • 7
    • 7
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Tyr88

    Specificity

    This antibody detects endogenous levels of total Cofilin-1/2 protein.

    Cross-Reactivity (Details)

    Also reacts to: Cofilin 2

    Purification

    Immunoaffinity Chromatography.

    Immunogen

    The antiserum was produced against synthesized non-phosphopeptide derived from human Cofilin1/Cofilin2 around the phosphorylation site of Tyrosine 88 (A-T-Yp-E-T).
  • Application Notes

    Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    -20 °C

    Storage Comment

    Store the antibody (in aliquots) at-20 °C.
  • Target

    Cofilin (CFL1) (Cofilin 1 (CFL1))

    Alternative Name

    Cofilin-1

    Background

    Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18

    Gene ID

    1072

    NCBI Accession

    NP_005498

    UniProt

    P23528

    Pathways

    Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
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