Cofilin antibody (Tyr88)
Quick Overview for Cofilin antibody (Tyr88) (ABIN319381)
Target
See all Cofilin (CFL1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Tyr88
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Specificity
- This antibody detects endogenous levels of total Cofilin-1/2 protein.
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Cross-Reactivity (Details)
- Also reacts to: Cofilin 2
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Purification
- Immunoaffinity Chromatography.
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Immunogen
- The antiserum was produced against synthesized non-phosphopeptide derived from human Cofilin1/Cofilin2 around the phosphorylation site of Tyrosine 88 (A-T-Yp-E-T).
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Application Notes
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Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Store the antibody (in aliquots) at-20 °C.
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- Cofilin (CFL1) (Cofilin 1 (CFL1))
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Alternative Name
- Cofilin-1
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Background
- Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18
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Gene ID
- 1072
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NCBI Accession
- NP_005498
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UniProt
- P23528
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Pathways
- Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
Target
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