Neutrophils antibody

Catalog No. ABIN319511

The Rat Monoclonal anti-Neutrophils antibody is suitable to detect Neutrophils in samples from Mouse. It has been validated for FACS, WB, IHC (fro) and IHC (p).

Quick Overview for Neutrophils antibody (ABIN319511)

Target: Neutrophils

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Neutrophils antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 07-04-14

Product Details anti-Neutrophils Antibody

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Endotoxin Level: Low Endotoxin

Immunogen: Cultured bone marrow cells. Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: use 10 μL of 1/50-1/100 to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Sections. Western blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Neutrophils

Target: Neutrophils