Integrin beta 3 antibody

Catalog No. ABIN319518

This anti-Integrin beta 3 antibody is a Mouse Monoclonal antibody detecting Integrin beta 3 in WB, FACS, IHC (fro) and IHC (p). Suitable for Human.

Quick Overview for Integrin beta 3 antibody (ABIN319518)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Y2-51

Product Details anti-Integrin beta 3 Antibody

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: PHA stimulated peripheral blood cells. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Sections: This product requires proteindigestion pre-treatment of paraffin sections e. g. trypsin prior to staining. Western Blotting: Detects a band/s of approximately 90 kDa in platelet lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61 / ITGB3

Background: CD61 is a 105kD glycoprotein, which is also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.Synonyms: GP3A, GPIIIa, Integrin beta-3, Platelet membrane glycoprotein IIIa

Gene ID: 9606

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex