TCR gamma/delta antibody

Catalog No. ABIN319644

The Hamster Monoclonal anti-TCR gamma/delta antibody (Clone GL3) (ABIN319644) specifically detects TCR gamma/delta in FACS, IP and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for TCR gamma/delta antibody (ABIN319644)

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This TCR gamma/delta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: GL3

Product Details anti-TCR gamma/delta Antibody

Purification: Affinity Chromatography

Immunogen: C57BL/6J intra epithelial lymphocytes. Remarks: Spleen cells from immunised Armenian hamster were fused with cells of the murine myeloma SP2/0 myeloma cell line.

Isotype: IgG

Application Details

Application Notes: Flow cytometry: 1:2 - 1:20, use 10 μL of diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Alternative Name: T Cell Receptor / TCR gamma/delta

Background: T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The TCR gamma/delta heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains. It is expressed during thymopoiesis and on the small subpopulation of gamma/delta TCR expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are gamma/delta+ and have limited receptor diversity. The TCR gamma/delta heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation.Synonyms: T-Cell Receptor delta, T-Cell Receptor gamma, T-Cell Receptor gamma delta, TCRD, TCRG