NOS1 antibody (AA 1422-1433)

Catalog No. ABIN3197502

This anti-NOS1 antibody is a Rabbit Polyclonal antibody detecting NOS1 in WB, IP, IF and IHC (p). Suitable for Human and Rat.

Quick Overview for NOS1 antibody (AA 1422-1433) (ABIN3197502)

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NOS1 Antibody

Binding Specificity: AA 1422-1433

Sequence: ESKKDTDEVF SS

Specificity: This antibody is specific to Nitric-oxide Synthase, Brain (NOS1/nNOS). Predicted

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to the Amino acids 1422-1433 of Human nNOS.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Immunocytochemistry. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: TBS, pH 7.4 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for NOS1

Target: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Alternative Name: NOS1

Background: Three isoforms of nitric oxide synthase (NOS) have been identified. All are homodimers with subunits of 130-160 kDa. All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain). The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site. Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production. NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO). Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane. In response to increased intracellular Ca2+, nNOS interacts with CaM. The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm. The dephosphorylation of nNOS by calcineurin initiates the production NO. NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways. nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).Synonyms: Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, Nitric Oxide Synthase, bNOS, nNOS

Gene ID: 4842

NCBI Accession: NP_000611

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction