ITGA2 antibody
Quick Overview for ITGA2 antibody (ABIN319775)
Target
See all ITGA2 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
- This antibody is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
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No Cross-Reactivity
- Rat (Rattus)
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Cross-Reactivity (Details)
- Species reactivity (tested):Human.
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Purification
- Affinity Chromatography on Protein G
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Immunogen
- Purified human beta1 preparation from HT1080 fibrosarcoma cell extract. Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
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Isotype
- IgG1
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Application Notes
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Flow Cytometry: 30 μg/mL, Immunoprecipitation: 25-50 μg/mL. Immunohistochemistry on Frozen Sections: 30-40 μg/mL. ELISA: 10 μg/mL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ITGA2 (Integrin alpha 2 (ITGA2))
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Alternative Name
- CD49b / ITGA2
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Background
- CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin alpha-2 chain. CD49b associates noncovalently with CD29, the integrin beta-1 subunit, to form the heterodimer integrin a2b1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts and long-term cultured T cells. CD49b/CD29 mediates the Mg++ dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during would healing.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2
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Gene ID
- 3673
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NCBI Accession
- NP_002194
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UniProt
- P17301
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Pathways
- CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex
Target
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