CD81 antibody

Catalog No. ABIN319907

The Hamster Monoclonal anti-CD81 antibody has been validated for FACS, WB, IP, IHC (fro) and EIA. It is suitable to detect CD81 in samples from Mouse and Rat. There is 1 publication available.

Quick Overview for CD81 antibody (ABIN319907)

Target: CD81

Reactivity: Mouse, Rat

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: Eat2

Product Details anti-CD81 Antibody

Purification: Affinity Chromatography on Protein G.

Immunogen: 38C13, murine B cell line

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL). Immunoprecipitation. Immunohistochemistry on Frozen Sections. ELISA. Western Blot. Not suitable for Paraffin Embedded Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD81 antibody (ABIN319907)

Blumenthal, Giebel, Ummanni, Schlüter, Endlich, Endlich: "Morphology and migration of podocytes are affected by CD151 levels." in: American journal of physiology. Renal physiology, Vol. 302, Issue 10, pp. F1265-77, (2012) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81 / TAPA1

Background: In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Synonyms: 26 kDa cell surface protein TAPA-1, Target of the antiproliferative antibody 1

Gene ID: 10090

UniProt: P35762

Pathways: Inositol Metabolic Process, Hepatitis C