CD3 antibody (Cytoplasmic Domain) (FITC)

Catalog No. ABIN320000

The Rat Monoclonal anti-CD3 antibody has been validated for FACS. It is suitable to detect CD3 in samples from Human, Chicken, Monkey, Pig, Dog, Horse and Cow. There is 1 publication available.

Quick Overview for CD3 antibody (Cytoplasmic Domain) (FITC) (ABIN320000)

Target: CD3

Reactivity: Human, Chicken, Monkey, Pig, Dog, Horse, Cow

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: CD3-12

Product Details anti-CD3 Antibody (FITC)

Binding Specificity: Cytoplasmic Domain

Sequence: Glu-Arg-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys

Specificity: This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. Cross reacts with Bovine, Dog, Horse, Rhesus Monkey, Pig, Mouse and Chicken.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Synthetic peptide sequence derived from cytoplasmic epitope of CD3.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/5-1/10 diluted antibody to label 10^6 cells in 100 μL. Membrane permeabilisation is required. We recommend use of Leucoperm for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD3 antibody (FITC) (ABIN320000)

Shrestha, Hashiguchi, Ito, Miura, Takenouchi, Oyama, Kawahara, Tancharoen, Ki-I, Arimura, Yoshinaga, Noma, Shrestha, Nitanda, Kitajima, Arimura, Sato, Sakamoto, Maruyama: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway