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CD16 antibody

CD16 Reactivity: Human FACS, IHC (fro) Host: Rat Monoclonal YFC120-5 unconjugated

Catalog No. ABIN320001

Datasheet as PDF

Target See all CD16 Antibodies

CD16
Reactivity
133

29

20

20

20

9

8

8

8

4

3

3

2

2

2

2

2

2

2

2

2

2
Human
Host
115

26

20

1
Rat
Compatible Secondaries
Clonality
136

24

1
Monoclonal
Conjugate
64

18

14

13

7

5

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD16 antibody is un-conjugated
Application
133

36

28

28

26

24

23

17

6

6

5

4

4

2

2

2

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

This antibody binds specifically to the leucocyte low affinity Fc receptor (Fc RIII), also known as CD16. It recognises a 50-70 kDa leucocyte surface antigen.

Purification

Affinity chromatography on Protein A.

Immunogen

Human neutrophils. Spleen cells from Lou rat fused with the rat myeloma cell line. Y3/Ag.1.2.3.

Clone

YFC120-5

Isotype

IgG2b

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CD16 Reactivity: Human FACS, IP, WB Host: Mouse Monoclonal MEM-154 Biotin

anti-CD16 (CD16) (AA 17-208) antibody
CD16 Reactivity: Human FACS, ELISA Host: Mouse Monoclonal 2G10A9 unconjugated

anti-CD16 (CD16) antibody
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Application Notes

Flow Cytometry. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD16

Alternative Name

CD16 (CD16 Products)

Background

The antigen is expressed by neutrophils, NK cells, K cells and a subpopulation of macrophages.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID

2214

NCBI Accession

NP_000560

UniProt

P08637

Pathways

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process