CD44 antibody

Catalog No. ABIN320086

This Mouse Monoclonal antibody specifically detects CD44 in FACS, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Monkey.

Quick Overview for CD44 antibody (ABIN320086)

Target: CD44

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: F10-44-2

Product Details anti-CD44 Antibody

Specificity: This antibody recognises the human CD44 cell surface antigen and is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Purification: Affinity Chromatography on Protein G.

Immunogen: Human T lymphocytes. Spleen cells of immunised BALB/c mice were fused with cells from the mouse NS1 myeloma line.

Isotype: IgG2a

Application Details

Application Notes: Suitable for Flow cytometry (Use 10 μL of neat antibody to label 10^6 cells in 100 μL). Immunohistochemistry on Paraffin Sections (1/50-1/100) and Frozen sections. Positive Control: human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: The human CD44 cell surface antigen is a 100 kD glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1

Gene ID: 960

NCBI Accession: NP_000601

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling