SLA (Haploid Sperm Cell-Specific Antigen) antibody

Catalog No. ABIN3201019

There are 3+ publications for this product available. The Rat Monoclonal anti-SLA (Haploid Sperm Cell-Specific Antigen) antibody is suitable to detect SLA (Haploid Sperm Cell-Specific Antigen) in samples from Mouse and Rat. It has been validated for IEM, IHC and WB.

Quick Overview for SLA (Haploid Sperm Cell-Specific Antigen) antibody (ABIN3201019)

Target: SLA (Haploid Sperm Cell-Specific Antigen)

Reactivity: Mouse, Rat

Host: Rat

Clonality: Monoclonal

Application: Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: TRA54

Product Details

Cross-Reactivity (Details): Not tested in other species.

Characteristics: The antibody was purified from the serum-free culture medium of the hybridoma clone TRA54 by proprietary chromatography processes under mild conditions.
This antibody can be used for immunohistochemical staining of testicular tissues. The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testes by the group of Prof. Yoshitake Nishimune of Osaka University.

Purification: Purified

Isotype: IgM

Application Details

Application Notes: 1. Western blotting: ~1 g/mL
2. Immunohistochemical staining
3. Immunoelectron microscopy
Not tested in other applications

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Preservative: Azide free

Storage: -20 °C

References

Arrotéia, Joazeiro, Yamada, Tanaka, Nishimune, Pereira: "Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens." in: Journal of andrology, Vol. 25, Issue 6, pp. 914-21, (2004) (PubMed).

Ventelä, Toppari, Parvinen: "Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing." in: Molecular biology of the cell, Vol. 14, Issue 7, pp. 2768-80, (2003) (PubMed).

Pereira, Tanaka, Nagata, Sawada, Mori, Chimelli, Nishimune: "Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cells." in: International journal of andrology, Vol. 21, Issue 1, pp. 34-40, (1998) (PubMed).

Target Details

Target: SLA (Haploid Sperm Cell-Specific Antigen)

Background: Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification. The molecular size diversity is due to the difference in glycosilation.