Clusterin antibody (AA 228-449)

Catalog No. ABIN3201572

The Mouse Monoclonal anti-Clusterin antibody (Clone 6#) (ABIN3201572) specifically detects Clusterin in WB, IHC, IP and ICC. The antibody is reactive with Human samples.

Quick Overview for Clusterin antibody (AA 228-449) (ABIN3201572)

Target: Clusterin (CLU)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 6#

Product Details anti-Clusterin Antibody

Binding Specificity: AA 228-449

Purpose: Monoclonal Antibody to Clusterin (CLU)

Sequence: MGHHHHHHSG S-SLM PFSPYEPLNF HAMFQPFLEM IHEAQQAMDI HFHSPAFQHP PTEFIREGDD DRTVCREIRH NSTGCLRMKD QCDKCREILS VDCSTNNPSQ AKLRRELDES LQVAERLTRK YNELLKSYQW KMLNTSSLLE QLNEQFNWVS RLANLTQGED QYYLRVTTVA SHTSDSDVPS GVTEVVVKLF DSDPITVTVP VEVSRKNPKF METVAEKALQ EYRKKHREE

Specificity: The antibody is a mouse monoclonal antibody raised against CLU. It has been selected for its ability to recognize CLU in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Clusterin (CLU) corresdonding to Ser228~Glu449 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-6 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background: CLU, TRPM2, KUB1, CLI, APOJ, SGP2, AAG4, APO-J, SP-40, Apolipoprotein J, Complement Lysis Inhibitor, 40,Sulfated Glycoprotein 2, Testosterone-Repressed Prostate Message 2

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling