CD90 antibody

Catalog No. ABIN320170

This Rat Monoclonal antibody specifically detects CD90 in FACS, WB, IP and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for CD90 antibody (ABIN320170)

Target: CD90 (THY1)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: FF-10

Product Details anti-CD90 Antibody

Specificity: This antibody recognises the Thy1.2 cell surface antigen, and binds to T cell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognises a 30 kDa glycoprotein in Immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.

Purification: Affinity Chromatography on Protein G.

Immunogen: EL-4 cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells from the mouse SP2/0 - Ag14 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry (1/50-1/100). Immunohistochemistry on Frozen Sections. Immunoprecipitation. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90

Background: CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gene ID: 21838

NCBI Accession: NP_033408

UniProt: P01831

Pathways: Cell-Cell Junction Organization