CD52 antibody

Catalog No. ABIN320173

This Rat Monoclonal antibody specifically detects CD52 in FACS, WB, IHC (p), IHC (fro), CyTox, IP and EIA. It exhibits reactivity toward Human and Monkey.

Quick Overview for CD52 antibody (ABIN320173)

Target: CD52

Reactivity: Human, Monkey

Host: Rat

Clonality: Monoclonal

Conjugate: This CD52 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: YTH34-5

Product Details anti-CD52 Antibody

Specificity: SM2135LE reacts with the Human CD52 antigen, also known as CAMPATH-1. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Endotoxin Level: Low Endotoxin

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry (1/50-1/100): Use 10 μL to label 1 x 10^6 cells in 100 μL. Immunohistochemistry (Frozen/Paraffin/Resin): This product does not require protein digestion pretreatment of paraffin section. However enhanced results may be seen using Trypsin protein digestion (2). ELISA. Western Blotting. Immunoprecipitation. Cytotoxic Assays (50 μg/mL): Use human serum as complement source.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives or stabilizers.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD52

Target: CD52

Alternative Name: CD52 / CAMPATH-1

Background: The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.Synonyms: CDw52, Cambridge pathology 1 antigen, Epididymal secretory protein E5, HE5

Gene ID: 1043

NCBI Accession: NP_001794

UniProt: P31358