MADCAM1 antibody

Catalog No. ABIN320242

This Mouse Monoclonal antibody specifically detects MADCAM1 in FACS, IHC (p) and EIA. It exhibits reactivity toward Human and has been mentioned in 6+ publications.

Quick Overview for MADCAM1 antibody (ABIN320242)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MADCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 314G8

Product Details anti-MADCAM1 Antibody

Specificity: This antibody recognises Mucosal Addressin Cell Adhesion Molecule 1 (MAdCAM-1). MAdCAM-1 expression has also been reported in the gut lamina propria but Clone 314G8 does not recognise MAdCAM-1 in these tissues. Clone 314G8 reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7. We recommend the use of SM2237LE for this purpose.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein. Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA. Western Blot: under reducing conditions, This antibody detects a band of approximately 60 kDa. Flow Cytometry: 1/10- 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on paraffin embedded sections: 1/50, This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-MADCAM1 antibody (ABIN320242)

Pardanani, Lasho, Chen, Kimlinger, Finke, Zblewski, Patnaik, Reichard, Rowinsky, Hanson, Brooks, Tefferi: "Aberrant expression of CD123 (interleukin-3 receptor-α) on neoplastic mast cells." in: Leukemia, Vol. 29, Issue 7, pp. 1605-8, (2015) (PubMed).

Rancourt, Veress, Guo, Jones, Hendry-Hofer, White: "Airway tissue factor-dependent coagulation activity in response to sulfur mustard analog 2-chloroethyl ethyl sulfide." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 302, Issue 1, pp. L82-92, (2012) (PubMed).

Olloquequi, Montes, Prats, Rodríguez, Montero, García-Valero, Ferrer: "Significant increase of CD57+ cells in pulmonary lymphoid follicles of COPD patients." in: The European respiratory journal, Vol. 37, Issue 2, pp. 289-98, (2011) (PubMed).

Leung, Lehnert, Kanwar, Yang, Mon, McNeil, Krissansen: "Bioassay detects soluble MAdCAM-1 in body fluids." in: Immunology and cell biology, Vol. 82, Issue 4, pp. 400-9, (2004) (PubMed).

Eipel, Bordel, Nickels, Menger, Vollmar: "Impact of leukocytes and platelets in mediating hepatocyte apoptosis in a rat model of systemic endotoxemia." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 286, Issue 5, pp. G769-76, (2004) (PubMed).

Slavícková, Dráber, Dráberová, Dráber, Viklický: "A novel panel of monoclonal antibodies against beta-galactosidase of Escherichia coli and its versatility for detection of recombinant expression products." in: Folia biologica, Vol. 38, Issue 6, pp. 350-7, (1993) (PubMed).

Target Details for MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Alternative Name: MAdCAM1

Background: MAdCAM-1 is a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria.Synonyms: MAdCAM-1, Mucosal addressin cell adhesion molecule 1, hMAdCAM-1

Gene ID: 8174

NCBI Accession: NP_570116

UniProt: Q13477