CD147 antibody (PE)

Catalog No. ABIN320330

The Mouse Monoclonal anti-CD147 antibody is suitable to detect CD147 in samples from Rat. It has been validated for FACS.

Quick Overview for CD147 antibody (PE) (ABIN320330)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD147 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: OX-47

Product Details anti-CD147 Antibody (PE)

Specificity: This antibody recognises a membrane glycoprotein of MW 42-48 kDa. Clone OX-47 is reported to be suitable for use in Western blotting. (4)

Purification: Affinity Chromatography on Protein G.

Immunogen: T blasts from a mixed lymphocyte reaction. Spleen cells from immunised BALB/c mice were fused with cells from the NS0/1 mouse myeloma cell line

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted Prior to and after reconstitution at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.Reconstitution: Restore with 1.0 ml distilled water..

Expiry Date: 12 months

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: CD147

Background: OX-47 is expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen is also present on some endothelia. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue. It is abundant on activated lymphocytes: it is the homologue of the chicken blood brain barrier antigen HT7.(2)Synonyms: 5F7, BSG, Basigin, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, OK blood group antigen, TCSF, Tumor cell-derived collagenase stimulatory factor

Gene ID: 25246

UniProt: P26453

Pathways: S100 Proteins