PUF60 antibody (AA 371-420)

Catalog No. ABIN320588

The Rabbit Polyclonal anti-PUF60 antibody has been validated for WB, IHC and IHC (p). It is suitable to detect PUF60 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig and Monkey.

Quick Overview for PUF60 antibody (AA 371-420) (ABIN320588)

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUF60 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PUF60 Antibody

Binding Specificity: AA 371-420

Specificity: Human PUF60

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Guinea pig (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa371-420 of human PUF60 (Q9UHX1-2, NP_055096). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Opossum, Guinea pig (100%), Orangutan, Bat (92%), Turkey, Zebra finch, Chicken, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Alternative Name: PUF60

Background: Name/Gene ID: PUF60

Synonyms: PUF60, FBP-interacting repressor, FBP interacting repressor, RoBPI, Siah-BP1, Ro-binding protein 1, RoBP1, Siah binding protein 1, FIR, Siah-binding protein 1, SIAHBP1

Gene ID: 22827

NCBI Accession: NP_055096

UniProt: Q9UHX1