KRT20 antibody (AA 181-230)

Catalog No. ABIN320732

The Rabbit Polyclonal anti-KRT20 antibody has been validated for WB. It is suitable to detect KRT20 in samples from Human, Mouse, Rat and Dog.

Quick Overview for KRT20 antibody (AA 181-230) (ABIN320732)

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KRT20 Antibody

Binding Specificity: AA 181-230

Specificity: Human KRT20 / Cytokeratin 20

Predicted Reactivity: Percent identity by BLAST analysis: Rabbit (92%) Dog (84%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa181-230 of human KRT20 (P35900, NP_061883). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat (100%), Marmoset, Bat, Rabbit (92%), Pig (85%), Dog, Horse (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: KRT20 / CK20 / Cytokeratin 20

Background: Name/Gene ID: KRT20
Family: Keratin

Synonyms: KRT20, Cytokeratin-20, Cytokeratin 20, CK20, K20, Keratin 20, Keratin-20, KRT21, CK-20, Protein IT

Gene ID: 54474

NCBI Accession: NP_061883

UniProt: P35900