NOV antibody (AA 231-280)

Catalog No. ABIN320813

The Rabbit Polyclonal anti-NOV antibody has been validated for WB. It is suitable to detect NOV in samples from Human, Mouse, Cow, Dog, Guinea Pig, Rabbit and Monkey.

Quick Overview for NOV antibody (AA 231-280) (ABIN320813)

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOV antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NOV Antibody

Binding Specificity: AA 231-280

Specificity: Human NOV / CCN3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa231-280 of human NOV (P48745, NP_002505). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%), Mouse, Bat, Horse (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: CCN3 / NOV

Background: Name/Gene ID: NOV

Synonyms: NOV, CCN3, CCN family member 3, IGFBP9, IBP-9, IGFBP-9, NOVh, IGF-binding protein 9, Nephroblastoma overexpressed, Protein NOV homolog

Gene ID: 4856

NCBI Accession: NP_002505

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding