IL-10RA antibody (AA 128-177)

Catalog No. ABIN320912

The Rabbit Polyclonal anti-IL-10RA antibody has been validated for WB. It is suitable to detect IL-10RA in samples from Human, Cow, Pig, Horse and Monkey. There is 1 publication available.

Quick Overview for IL-10RA antibody (AA 128-177) (ABIN320912)

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Reactivity: Human, Cow, Pig, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-10RA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IL-10RA Antibody

Binding Specificity: AA 128-177

Specificity: Human IL10RA

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (92%) Guinea pig (91%) Horse (84%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa128-177 of human IL10RA (Q13651, NP_001549). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Galago, Panda, Dog (92%), Guinea pig (91%), Horse (84%), Platypus (83%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-IL-10RA antibody (ABIN320912)

Mallikarjunappa, Shandilya, Sharma, Lamers, Bissonnette, Karrow, Meade: "Functional analysis of bovine interleukin-10 receptor alpha in response to Mycobacterium avium subsp. paratuberculosis lysate using CRISPR/Cas9." in: BMC genetics, Vol. 21, Issue 1, pp. 121, (2020) (PubMed).

Target Details for IL-10RA

Target: IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))

Alternative Name: IL10RA

Background: Name/Gene ID: IL10RA
Family: Interleukin

Synonyms: IL10RA, CD210, CD210a, CD210 antigen, IBD28, IL-10R subunit 1, Il-10r, IL-10R subunit alpha, Interleukin 10 receptor, alpha, IL10R, Interleukin-10 receptor, HIL-10R, CDW210A, IL-10 receptor subunit alpha, IL-10R1, IL-10RA, Interleukin-10 receptors

Gene ID: 3587

NCBI Accession: NP_001549

UniProt: Q13651

Pathways: Growth Factor Binding