ALT antibody (AA 31-80)

Catalog No. ABIN320978

The Rabbit Polyclonal anti-ALT antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ALT in samples from Human, Rat, Mouse, Pig, Dog, Cow, Guinea Pig, Hamster, Monkey and Bat.

Quick Overview for ALT antibody (AA 31-80) (ABIN320978)

Target: ALT (Alanine Aminotransferase (ALT))

Reactivity: Human, Rat, Mouse, Pig, Dog, Cow, Guinea Pig, Hamster, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ALT Antibody

Binding Specificity: AA 31-80

Specificity: Human GPT / ALT1

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Dog, Pig, Guinea pig (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa31-80 of human GPT (P24298, NP_005300). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Dog, Bovine, Bat, Pig, Guinea pig (100%), Elephant, Opossum, Platypus, Xenopus, Salmon (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ALT

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: GPT / Alanine Transaminase

Background: Name/Gene ID: GPT

Synonyms: GPT, AAT1, ALT1, Alanine aminotransferase, Alanine aminotransferase 1, GPT 1, GPT1

Gene ID: 2875

NCBI Accession: NP_005300

UniProt: P24298