Estrogen Receptor alpha antibody (Estrogen Receptor 1) (AA 410-592)

Details for Product anti-ESR1 Antibody No. ABIN3210000
Target
Binding Specificity
AA 410-592
43
31
29
27
26
24
23
21
21
18
17
16
16
16
15
15
15
13
11
11
10
8
7
6
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
594
255
206
40
33
12
10
9
7
7
5
5
4
3
3
1
1
Host
Mouse
453
177
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Estrogen Receptor alpha antibody is un-conjugated
42
24
24
17
16
13
12
12
12
12
12
11
6
6
6
6
6
4
4
4
4
4
4
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
446
212
207
143
103
80
79
55
55
18
16
16
13
13
11
9
7
3
2
1
1
1
1
1
1
1
1
1
1
1
Options
Immunogen ER alpha antibody was raised in Mouse using a purified recombinant fragment of human ER-alpha (410-592 aa) expressed in E. coli as the immunogen.
Clone 2B7
Isotype IgG1
Purification Purified antibody.
Target
Alternative Name ER alpha (ESR1 Antibody Abstract)
Background The estrogen receptor (ESR) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ESR1 mRNA transcripts, which differ primarily in their 5-prime untranslated regions. The translated receptors show less variability. Synonyms: Monoclonal ER alpha antibody, Anti-ER alpha antibody, Estrogen receptor alpha antibody, ERA antibody, NR3A1 antibody.
Pathways Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
Application Notes ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.
Restrictions For Research Use only
Concentration Lot specific
Buffer Purified antibody supplied in PBS containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage RT/-20 °C
Storage Comment Store at 4 °C for short term storage. Store at -20 °C for long term storage.
Images
Image no. 1 for anti-Estrogen Receptor 1 (ESR1) (AA 410-592) antibody (ABIN3210000) Western Blot showing ER-alpha antibody used against human breast carcinoma tissue lys...
Image no. 2 for anti-Estrogen Receptor 1 (ESR1) (AA 410-592) antibody (ABIN3210000) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-al...