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GFP antibody

Name: GFP antibody
Brand: antibodies-online
SKU: ABIN3210020
Price: 961.13 USD
Availability: InStock

GFP Reactivity: Aequorea victoria WB, ELISA, IHC, FACS Host: Mouse Monoclonal 4B10B2 unconjugated

Western Blot showing using GFP antibody used against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).

FC analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP antibody .

FACS

Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP antibody .

IHC

Western Blot showing GFP antibody used against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).

4 images

Catalog No. ABIN3210020

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
175

19

15

15

12

7

5

4

2

1

1

1

1
Aequorea victoria
Host
74

73

35

11

9

6

2

1
Mouse
Compatible Secondaries
Clonality
121

85
Monoclonal
Conjugate
104

14

12

9

6

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
173

87

55

42

33

31

29

21

16

14

14

14

12

6

4

4

3

3

2

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification

Purified

Immunogen

GFP antibody was raised in Mouse using a purified recombinant fragment of GFP expressed in E. coli as the immunogen.

Clone

4B10B2

Isotype

IgG2a

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Application Notes

ELISA: 1:10,000, FC: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Purified antibody supplied in PBS containing 0.03 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C for short term storage. Store at -20 °C for long term storage.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Target Type

Viral Protein

Background

Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry. Synonyms: Monoclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody.