DKK1 antibody (C-Term)

Catalog No. ABIN321363

This anti-DKK1 antibody is a Rabbit Polyclonal antibody detecting DKK1 in WB. Suitable for Human, Rat, Dog, Cow, Horse, Rabbit, Goat, Guinea Pig, Zebrafish (Danio rerio), Pig, Monkey, Bat and Xenopus laevis.

Quick Overview for DKK1 antibody (C-Term) (ABIN321363)

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivity: Human, Rat, Dog, Cow, Horse, Rabbit, Goat, Guinea Pig, Zebrafish (Danio rerio), Pig, Monkey, Bat, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DKK1 Antibody

Binding Specificity: C-Term

Specificity: Human DKK1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Goat, Rabbit, Horse, Pig, Guinea pig, Xenopus (100%) Mouse (92%) Chicken, Zebrafish (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human DKK1 (O94907, NP_036374). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Rat, Goat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Platypus, Xenopus (100%), Mouse, Hamster, Opossum (92%), Turkey, Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for DKK1

Target: DKK1 (Dickkopf Homolog 1 (DKK1))

Alternative Name: DKK1

Background: Name/Gene ID: DKK1

Synonyms: DKK1, Dickkopf-1 like, DKK-1, Dickkopf-1, Dickkopf-related protein 1, Dickkopf related protein-1, HDkk-1, Hdkk1, Dickkopf-like protein 1, SK

Gene ID: 22943

NCBI Accession: NP_036374

UniProt: O94907

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation