PDHA1 antibody (N-Term)

Catalog No. ABIN321377

The Rabbit Polyclonal anti-PDHA1 antibody has been validated for WB. It is suitable to detect PDHA1 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Monkey and Bat.

Quick Overview for PDHA1 antibody (N-Term) (ABIN321377)

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDHA1 Antibody

Binding Specificity: N-Term

Specificity: Human PDHA1 / PDHA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Guinea pig (100%) Bovine (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide from N-Terminus of human PDHA1 (P08559, NP_000275). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Guinea pig (100%), Hamster, Bovine, Horse, Pig, Platypus (92%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDHA1

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Alternative Name: PDHA1 / PDH E1 alpha

Background: Name/Gene ID: PDHA1

Synonyms: PDHA1, PDHCE1A, PDHA, PHE1A, PDHE1-A type I

Gene ID: 5160

NCBI Accession: NP_000275

UniProt: P08559

Pathways: Warburg Effect