eEF1A1 antibody (C-Term)

Catalog No. ABIN321389

The Rabbit Polyclonal anti-eEF1A1 antibody has been validated for WB. It is suitable to detect eEF1A1 in samples from Human, Mouse, Rat, Cow, Chicken, Dog, Sheep, Rabbit, Horse, Guinea Pig, Goat, Monkey, Bat, Hamster, Pig and Xenopus laevis.

Quick Overview for eEF1A1 antibody (C-Term) (ABIN321389)

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Sheep, Rabbit, Horse, Guinea Pig, Goat, Monkey, Bat, Hamster, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eEF1A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-eEF1A1 Antibody

Binding Specificity: C-Term

Specificity: Human EEF1A1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Goat, Rabbit, Pig, Guinea pig, Chicken (100%) Zebrafish (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human EEF1A1 (P68104, NP_001393). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Sheep, Goat, Hamster, Dog, Cat, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Xenopus (100%), Elephant, Panda, Lizard, Salmon, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for eEF1A1

Target: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Alternative Name: EEF1A1 / EEF1A

Background: Name/Gene ID: EEF1A1

Synonyms: EEF1A1, Cervical cancer suppressor 3, CCS-3, CCS3, EEF-1, Elongation factor Tu, EEF1A, EF-Tu, EF1A, Elongation factor 1-alpha 1, GRAF-1EF, LENG7, PTI1, CTCL tumor antigen, EE1A1, EEF1A-1, EF-1-alpha-1, EF1a-like protein, HNGC:16303

Gene ID: 1915

NCBI Accession: NP_001393

UniProt: P68104