ATP5B antibody (AA 131-180)

Catalog No. ABIN321395

The Rabbit Polyclonal anti-ATP5B antibody has been validated for WB, IHC and IHC (p). It is suitable to detect ATP5B in samples from Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Hamster and Bat.

Quick Overview for ATP5B antibody (AA 131-180) (ABIN321395)

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Hamster, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATP5B Antibody

Binding Specificity: AA 131-180

Specificity: Human ATP5B

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Rabbit, Horse, Pig (100%) Xenopus, Zebrafish (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa131-180 of human ATP5B (P06576, NP_001677). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Cat, Bovine, Bat, Rabbit, Horse, Pig, Zebra finch (100%), Monkey, Chicken, Lizard, Xenopus, Salmon, Zebrafish (92%), Drosophila (91%), Stickleback (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATP5B

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Alternative Name: ATP5B

Background: Name/Gene ID: ATP5B
Subfamily: ATPase - F type
Family: Transporter

Synonyms: ATP5B, ATPMB, ATPSB, Beta-mtATPase, F0F1-ATP synthase beta subunit, ATP synthase beta subunit

Gene ID: 506

NCBI Accession: NP_001677

UniProt: P06576

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process