ATP5B antibody (AA 135-163)

Catalog No. ABIN650786

The Rabbit Polyclonal anti-ATP5B antibody is suitable to detect ATP5B in samples from Human. It has been validated for WB, IF, FACS and IHC (p).

Quick Overview for ATP5B antibody (AA 135-163) (ABIN650786)

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB20513

Product Details anti-ATP5B Antibody

Binding Specificity: AA 135-163

Predicted Reactivity: B, M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATP5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-163 amino acids from the Central region of human ATP5B.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:25. IHC-P: 1:25. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for ATP5B

Target: ATP5B (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, beta Polypeptide (ATP5B))

Alternative Name: ATP5B

Background: ATP5B is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). It is the beta subunit of the catalytic core.

Molecular Weight: 56560

Gene ID: 506

NCBI Accession: NP_001677

UniProt: P06576

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process