ENO3 antibody (N-Term)

Catalog No. ABIN321399

This anti-ENO3 antibody is a Rabbit Polyclonal antibody detecting ENO3 in WB, IHC and IHC (p). Suitable for Human, Mouse, Rat, Rabbit, Cow, Dog, Goat, Guinea Pig, Horse, Monkey and Pig.

Quick Overview for ENO3 antibody (N-Term) (ABIN321399)

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Goat, Guinea Pig, Horse, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ENO3 Antibody

Binding Specificity: N-Term

Specificity: Human ENO3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Dog, Rabbit, Pig (100%) Xenopus, Zebrafish (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide from N-Terminus of human ENO3 (P13929, NP_001967). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset, Mouse, Rat, Goat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%), Orangutan, Gibbon, Galago, Hamster, Bat, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Catfish, Salmon, Smelt, Zebrafish, Blood fluke, Nematode, Grape, Toxoplasma, Arabidopsis, Trichina worm (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ENO3

Target: ENO3 (Enolase 3 (Beta, Muscle) (ENO3))

Alternative Name: ENO3 / Enolase 3

Background: Name/Gene ID: ENO3

Synonyms: ENO3, Beta-enolase, Enolase 3, Enolase 3 (beta, muscle), Enolase 3, (beta, muscle), Muscle-specific enolase, MSE, Skeletal muscle enolase, GSD13

Gene ID: 2027

NCBI Accession: NP_001967

UniProt: P13929