PCMT1 antibody (AA 209-258)

Catalog No. ABIN321494

The Rabbit Polyclonal anti-PCMT1 antibody is suitable to detect PCMT1 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Bat, Chicken, Monkey, Pig and Xenopus laevis. It has been validated for WB.

Quick Overview for PCMT1 antibody (AA 209-258) (ABIN321494)

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Bat, Chicken, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PCMT1 Antibody

Binding Specificity: AA 209-258

Specificity: Human PCMT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig, Chicken, Xenopus (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa209-258 of human PCMT1 (P22061, NP_005380). Percent identity by BLAST analysis: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Xenopus (100%), Horse, Frog, Salmon, Smelt, Stickleback, Zebrafish (92%), Catfish (91%), Rice, Grape, Arabidopsis (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PCMT1

Target: PCMT1 (Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1))

Alternative Name: PCMT1

Background: Name/Gene ID: PCMT1

Synonyms: PCMT1, PIMT

Gene ID: 5110

NCBI Accession: NP_005380

UniProt: P22061

Pathways: Ribonucleoprotein Complex Subunit Organization