PPIA antibody (C-Term)

Catalog No. ABIN321858

The Rabbit Polyclonal anti-PPIA antibody (ABIN321858) specifically detects PPIA in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow, Pig, Monkey, Dog, Goat, Guinea Pig, Horse, Rabbit and Hamster samples.

Quick Overview for PPIA antibody (C-Term) (ABIN321858)

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey, Dog, Goat, Guinea Pig, Horse, Rabbit, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PPIA Antibody

Binding Specificity: C-Term

Specificity: Human PPIA / Cyclophilin A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig (100%) Rabbit (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human PPIA (P62937, NP_066953). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Marmoset, Tamarin, Mouse, Rat, Goat, Hamster, Dog, Cat, Bovine, Horse, Pig, Guinea pig (100%), Opossum (92%), Panda, Rabbit, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PPIA

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Alternative Name: PPIA / Cyclophilin A

Background: Name/Gene ID: PPIA

Synonyms: PPIA, Cyclophilin, Cyclosporin A-binding protein, Cyclophilin A, PPIase A, Rotamase A, CYPH, T cell cyclophilin

Gene ID: 5478

NCBI Accession: NP_066953

UniProt: P62937