Carboxypeptidase A2 antibody

Catalog No. ABIN322590

This anti-Carboxypeptidase A2 antibody is a Mouse Monoclonal antibody detecting Carboxypeptidase A2 in WB, ELISA, IP and Neut. Suitable for Human.

Quick Overview for Carboxypeptidase A2 antibody (ABIN322590)

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Carboxypeptidase A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Product Details anti-Carboxypeptidase A2 Antibody

Specificity: Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5 % , canine 90 %, rat 87 %, mouse 86 % and chicken 70 %.

Purification: Protein G purified

Immunogen: NS0-derived recombinant human rhCPA2.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Western blot:1-2 μg/mL the detection limit is ~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold. Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50 % of the enzymatic activity was inhibited at ~1.5 μg/mL (IC50) under conditions in which the enzyme was. present at 0.1 μg/mL and the substrate concentration was 0.1 mM. Considering the. molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at. approximately 5:1 Molar ratio of the antibody to the enzyme. Immunoprecipitation: 25 μg/mL. Direct ELISA: 0.5-1.0 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 1 mL sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 1 mL sterile PBS.

Target Details for Carboxypeptidase A2

Target: Carboxypeptidase A2 (CPA2) (Carboxypeptidase A2 (Pancreatic) (CPA2))

Alternative Name: CPA2

Background: Name/Gene ID: CPA2
Subfamily: Metallopeptidase M14
Family: Exopeptidase

Synonyms: CPA2, Carboxypeptidase A2

Gene ID: 1358

UniProt: P48052