2B4 antibody (Extracellular Domain)

Catalog No. ABIN324659

The Goat Polyclonal anti-2B4 antibody has been validated for WB, ELISA, FACS, IHC and IF. It is suitable to detect 2B4 in samples from Mouse.

Quick Overview for 2B4 antibody (Extracellular Domain) (ABIN324659)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This 2B4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-2B4 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes mouse 2B4 extracellular domain.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rm2B4 extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, IF, IHC, WB

Usage: Direct ELISA, Western Blot, Flow Cytometry, Immunohistochemistry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 0.3 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rh2B4 and less than 1 % cross-reactivity with rhSLAM. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x10⁶ with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Immunohistochemistry: This antibody will detect 2B4 in cells and tissues. The working dilution is 5-15 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244

Background: Name/Gene ID: CD244

Synonyms: CD244, 2B4, H2B4, NAIL, NKR2B4, Nmrk, SLAMF4, CD244 antigen

Gene ID: 51744

UniProt: Q9BZW8