2B4 antibody (Extracellular Domain)

Catalog No. ABIN324659

Product Details anti-2B4 Antibody

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This 2B4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: Recognizes mouse 2B4 extracellular domain.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rm2B4 extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, IF, IHC, WB

Usage: Direct ELISA, Western Blot, Flow Cytometry, Immunohistochemistry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 0.3 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rh2B4 and less than 1 % cross-reactivity with rhSLAM. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse 2B4. The detection limit for rm2B4 is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x10⁶ with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Immunohistochemistry: This antibody will detect 2B4 in cells and tissues. The working dilution is 5-15 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244 (CD244 Products)

Background: Name/Gene ID: CD244

Synonyms: CD244, 2B4, H2B4, NAIL, NKR2B4, Nmrk, SLAMF4, CD244 antigen

Gene ID: 51744

UniProt: Q9BZW8