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CD73 antibody (AA 29-549)

This Sheep Polyclonal antibody specifically detects CD73 in WB, FACS and ELISA. It exhibits reactivity toward Mouse.
Catalog No. ABIN324847

Quick Overview for CD73 antibody (AA 29-549) (ABIN324847)

Target

See all CD73 (NT5E) Antibodies
CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity

  • 178
  • 69
  • 36
  • 19
  • 18
  • 16
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Host

  • 108
  • 90
  • 14
  • 2
  • 1
  • 1
Sheep

Clonality

  • 121
  • 94
  • 1
Polyclonal

Conjugate

  • 96
  • 19
  • 16
  • 7
  • 7
  • 5
  • 4
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  • 4
  • 4
  • 4
  • 4
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  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This CD73 antibody is un-conjugated

Application

  • 150
  • 117
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  • 55
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  • 26
  • 15
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  • 1
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Western Blotting (WB), Flow Cytometry (FACS), ELISA
  • Binding Specificity

    • 16
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    • 9
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    • 1
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    • 1
    • 1
    AA 29-549

    Specificity

    Recognizes mouse CD73.

    Purification

    Immunoaffinity purified

    Immunogen

    CHO cell-derived rmCD73. (aa 29 - 549).

    Type of Immunogen: Recombinant protein

    Isotype

    IgG
  • Application Notes

    Approved: ELISA (0.5 - 1 μg/mL), Flo, WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Direct ELISA: 0.5-1.0 μg/mL. Detection limit for rmCD73 is. ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rmCD73 is ~1 ng/lane under non-reducing and reducing conditions. Flow Cytometry (Murine splenocytes): 25 μg/mL. Add 100 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    PBS, 40-50 % glycerol

    Concentration

    Lot specific

    Buffer

    Lyophilized from PBS, 5 % trehalose.

    Handling Advice

    Aviod frequent freeze thaws.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
  • Target

    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

    Alternative Name

    NT5E / eNT / CD73

    Background

    Name/Gene ID: NT5E

    Synonyms: NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase, 5 nucleotidase (CD73), 5-NT, 5-nucleotidase, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, Ecto-5-nucleotidase, ENT, EN, NT, Purine 5-Prime-Nucleotidase, NT5, NTE

    Gene ID

    4907

    UniProt

    P21589

    Pathways

    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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