PD-1 antibody (Extracellular Domain)

Catalog No. ABIN325052

The Goat Polyclonal anti-PD-1 antibody has been validated for FACS, WB, IHC and ELISA. It is suitable to detect PD-1 in samples from Mouse.

Quick Overview for PD-1 antibody (Extracellular Domain) (ABIN325052)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PD-1 Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes mouse PD-1 extracellular domain. Shows approximately 50 % cross-reactivity with rhPD-1.

Purification: Immunoaffinity purified

Immunogen: Sf21-derived rmPD-1 extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, IHC, WB

Usage: Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-1 in cells and tissues. The working dilution is 2-15 μg/mL. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x10⁶ with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 0.03 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handling Advice: Avoid frequent freeze thaws. Store undiluted.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PDCD1 / CD279 / PD-1

Background: Name/Gene ID: PDCD1
Family: Apoptosis

Synonyms: PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLEB2, PD-1, CD279 antigen, Programmed cell death 1

Gene ID: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints