PDCD1LG2 antibody

Catalog No. ABIN325068

This anti-PDCD1LG2 antibody is a Goat Polyclonal antibody detecting PDCD1LG2 in WB, FACS, IHC, ELISA and Func. Suitable for Mouse.

Quick Overview for PDCD1LG2 antibody (ABIN325068)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PDCD1LG2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Functional Studies (Func)

Product Details anti-PDCD1LG2 Antibody

Specificity: Recognizes mouse PD-L2. In direct ELISAs and Western Blots, this antibody shows ~20 % cross-reactivity with rhPD-L2.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmPD-L2.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo, Func, IHC, WB

Usage: Western Blot, Immunohistochemistry, Flow Cytometry, Blocking of receptor-ligand interaction, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-L2 in cells and tissues. The working dilution is 2-15 μg/mL. Flow Cytometry: This antibody has been tested on Tim-1 stimulated RAW 264.7 cells for use in flow cytometry. Dilute this antibody to 50 μg/mL and add 10 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Blocking of receptor-ligand interaction: Approximately 1-4 μg/mL of this antibody will block 50 % of the binding of 1 μg/mL of rmPD-1 to immobilized rmPD-L2/Fc chimera (100 μL of a 1 μg/mL solution was coated in each well) in a functional ELISA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PD-L2. The detection limit for rmPD-L2 is approximately 1.2 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PDCD1LG2 / CD273 / PD-L2

Background: Name/Gene ID: PDCD1LG2

Synonyms: PDCD1LG2, B7 dendritic cell molecule, B7-DC, B7DC, BA574F11.2, CD273, Btdc, CD273 antigen, PD-L2, PDCD1 ligand 2, PD-1-ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, PD-1 ligand 2, PDCD1L2, PDL2

Gene ID: 80380

UniProt: Q9BQ51