PODXL antibody (Extracellular Domain)

Catalog No. ABIN325654

This Mouse Monoclonal antibody specifically detects PODXL in WB, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for PODXL antibody (Extracellular Domain) (ABIN325654)

Target: PODXL (Podocalyxin-Like (PODXL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PODXL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PODXL Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes human Podocalyxin. In Western Blots, this antibody does not cross-react with rmPodocalyxin or rhEndoglycan.

Purification: Protein G purified

Immunogen: NS0-derived rhPodocalyxin extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, ICC (10 μg/mL), WB

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Immunocytochemistry: 10 μg/mL using fixed cells. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Antibody binding may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: t 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhPodocalyxin is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Alternative Name: PODXL / Podocalyxin

Background: Name/Gene ID: PODXL

Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81

Gene ID: 5420

UniProt: O00592

Pathways: Tube Formation