SCARF1 antibody

Catalog No. ABIN325827

The Mouse Monoclonal anti-SCARF1 antibody (ABIN325827) specifically detects SCARF1 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for SCARF1 antibody (ABIN325827)

Target: SCARF1 (Acetyl LDL Receptor (SCARF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCARF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SCARF1 Antibody

Specificity: Recognizes human SREC-I.

Purification: Immunoaffinity purified

Immunogen: NS0-derived recombinant human SREC-I.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (0.25 μg/mL), WB (1 - 2 μg/mL)

Usage: Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondaryreagents to detect human SREC-I. The detection limit for rhSREC-Iis approximately 1 ng/well and 100 ng/mL under non-reducingand reducing conditons, respectively.Flow Cytometry: 0.25 μg/mL. Use 10 μL of the diluted antibody tostain 1x10e5 to 2.5 x 10e5 cells in a total reaction volume notexceeding 200 μL. A fluorochrome-labeled secondary antibodysuch a goat anti-mouse IgG may be used for detection.Western Blot: 1-2 μg/mL with the appropriate secondary reagentsto detect human SREC-1. Using a colorimetric detection system,the detection limit for rhSREC-1 is approximately 1 ng/lane and100 ng/lane under non-reducing and reducing conditions,respectively. Chemiluminescent detection will increase sensitivityby 5 to 50 fold.Optimal dilutions should be determined by each laboratory foreach application.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Supplied as a powder lyophilized from a solution containing PBS with 5 % trehalose.

Target Details for SCARF1

Target: SCARF1 (Acetyl LDL Receptor (SCARF1))

Alternative Name: SCARF1 / SREC

Background: Name/Gene ID: SCARF1

Synonyms: SCARF1, Acetyl LDL receptor, KIAA0149, SREC-I, SREC1, SREC

Gene ID: 8578

UniProt: Q14162