BrdU antibody

Catalog No. ABIN6756975

The Sheep Polyclonal anti-BrdU antibody has been validated for IHC, IF, IHC (p), IHC (fro), WB, ELISA and IP. It is suitable to detect BrdU in samples from .

Quick Overview for BrdU antibody (ABIN6756975)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Sheep

Clonality: Polyclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-BrdU Antibody

Specificity: Recognizes bromodeoxyuridine at ~307kD. Species cross-reactivity: all species.

Purification: Protein G purified

Immunogen: Bromodeoxyuridine coupled to HGG.

Type of Immunogen: Hapten

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P (1:25), IP, WB

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Fluorescent In Situ Hybridization (FISH) Assay. Western Blot: Band is observed at ~307kD. Immunohistochemistry: Frozen and paraffin sections. Enzymatic antigen retrieval and DNA acid denaturation required. Fluorescent In Situ Hybridization: 1:25, Use a rabbit anti-sheep AMCA secondary antibody. Note: Denature DNA sample first so base is accessible to BrdU antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 40 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU

Target Type: Chemical