CMA1 antibody (AA 20-247)

Catalog No. ABIN328216

This Mouse Monoclonal antibody specifically detects CMA1 in WB, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for CMA1 antibody (AA 20-247) (ABIN328216)

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CMA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-CMA1 Antibody

Binding Specificity: AA 20-247

Specificity: Recognizes human Chymase at ~30kD. No cross reactivity with r  mMCPT-1, r  mMCPT-6, r  mMCPT-7 or r  mMCPT-11.

Purification: Protein G purified

Immunogen: NS0-derived rhChymase. (aa 20 - 247).

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IP (25 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Direct ELISA: 0.5-1 μg/mL. Detection limit for rhChymase is ~1 ng/well. Western Blot: 1-2 μg/mL. Detection limit for rhChymase is. ~10 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25 μg/mL from conditioned cell culture medium.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Alternative Name: CMA1 / MCT1 / Chymase

Background: Name/Gene ID: CMA1
Subfamily: Serine S1
Family: Protease

Synonyms: CMA1, Chymase, Chymase, heart, Chymase, mast cell, CYH, Mast cell chymase, Mcpt5, MCT1, Mast cell protease 5, Mast cell protease I, Heart chymase, Alpha-chymase, Chymase 1, Chymase 1, mast cell, CYM

Gene ID: 1215

UniProt: P23946

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis