Mesothelin antibody

Catalog No. ABIN328471

This Rat Monoclonal antibody specifically detects Mesothelin in ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Mesothelin antibody (ABIN328471)

Target: Mesothelin (MSLN)

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Product Details anti-Mesothelin Antibody

Specificity: Recognizes human mesothelin. This antibody binds to mesothelin cleaved form which is the ~40 kDa cell membrane-associated glycosylphosphatidylinositol-linked glycoprotein. It is not expected to react with the soluble portion of the mesothelin precursor protein that is designated as the megakaryocyte potentiating factor.

Purification: Immunoaffinity purified

Immunogen: NS0-derived recombinant human mesothelin.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (2.5 μg/10 x10⁶)

Usage: Flow Cytometry: This antibody was validated for flow cytometry using Hela human cervical carcinoma cells. Use at 2.5 μg per 10e6 cells. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Mesothelin. The detection limit for rhMesothelin is approximately 5 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 200 μL sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: MSLN / Mesothelin

Background: Name/Gene ID: MSLN

Synonyms: MSLN, CAK1 antigen, MPF, SMRP, Mesothelin, CAK1

Gene ID: 10232

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process