MLX antibody (AA 197-298)

Catalog No. ABIN328581

This anti-MLX antibody is a Goat Polyclonal antibody detecting MLX in WB, ELISA and ICC. Suitable for Human.

Quick Overview for MLX antibody (AA 197-298) (ABIN328581)

Target: MLX (MAX-Like Protein X (MLX))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MLX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-MLX Antibody

Binding Specificity: AA 197-298

Specificity: Recognizes human MLX. Species sequence homology: 99 % identical to mouse and canine MLX.

Purification: Immunoaffinity purified

Immunogen: E. coli-derived recombinant human rhMLX (aa 197-298).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Western blot, Immunocytochemistry and ELISA. Western blot: 0.1-0.2 μg/mL. The detection limit is ~2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL will detect human MLX in serum stimulated W138 human fetal lung fibroblasts. Fix cells with PBS containing 4 % paraformaldehyde for 20 minutes at RT and block with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, incubate cells with diluted primary antibody for 3 hours at RT. followed by NorthernLights 557 donkey anti-goat lgG at RT for 1 hour. Between each step, wash cells with PBS containing 0.1 % BSA. Direct ELISA: 0.5-1.0 μg/mL. The detection limit is ~0.3 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized powder from PBS, 5 % trehalose. Reconstitute, 0.5 mL PBS to a concentration of 0.2 mg/mL.

Target Details for MLX

Target: MLX (MAX-Like Protein X (MLX))

Alternative Name: MLX / TCFL4

Background: Name/Gene ID: MLX

Synonyms: MLX, BHLHd13, BigMax protein, MXD7, MAD7, Max-like bHLHZip protein, TCFL4, MLX, MAX dimerization protein, Protein BigMax, Max-like protein X, Transcription factor-like 4

Gene ID: 6945

UniProt: Q9UH92