EGFR antibody (Extracellular Domain)

Catalog No. ABIN329285

The Rat Monoclonal anti-EGFR antibody has been validated for ELISA and FACS. It is suitable to detect EGFR in samples from Human.

Quick Overview for EGFR antibody (Extracellular Domain) (ABIN329285)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Clone: 423103

Product Details anti-EGFR Antibody

Binding Specificity: Extracellular Domain

Specificity: Recognizes human EGFR.

Purification: Protein G purified

Immunogen: NS0-derived rhEGFR extracellular domain.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL)

Usage: Suitable for use in Flow Cytometry and Direct ELISA. Flow Cytometry: 25 μg/mL. Use 10 μL of diluted solution to label 1-2.5x10^5 cells. Direct ELISA: 0.5-1 μg/mL. The detection limit is ~5 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS

Concentration: Lot specific

Buffer: Lyophilized, PBS, 5 % trehalose

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Reconstitute with sterile PBS. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins