Ficolin 2 antibody

Catalog No. ABIN329856

The Mouse Monoclonal anti-Ficolin 2 antibody (ABIN329856) specifically detects Ficolin 2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for Ficolin 2 antibody (ABIN329856)

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ficolin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ficolin 2 Antibody

Specificity: Recognizes human Ficolin-2. Does not cross-react with rhFicolin-3.

Purification: Protein G purified

Immunogen: NS0-derived rhFicolin-2.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, WB

Usage: Western Blot, Direct ELISA. Western Blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect human Ficolin-2. Using a colorimetric detection system, the detection limit for rhFicolin-2 is approximately 25 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection with Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human Ficolin-2. The detection limit for rhFicolin-2 is approximately 1 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.

Target Details for Ficolin 2

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Alternative Name: FCN2 / Ficolin-2

Background: Name/Gene ID: FCN2

Synonyms: FCN2, 37 kDa elastin-binding protein, EBP-37, FCNL, Ficolin B, Ficolin-beta, Hucolin, Ficolin-2, L-ficolin, p35, Serum lectin p35, Ficolin-B

Gene ID: 2220

UniProt: Q15485

Pathways: Complement System