TCP1 alpha/CCTA antibody (C-Term)

Catalog No. ABIN331511

This Rat Monoclonal antibody specifically detects TCP1 alpha/CCTA in WB, IHC, IP and ICC. It exhibits reactivity toward Mouse, Rat, Cow, Dog, Rabbit, Hamster and Sheep.

Quick Overview for TCP1 alpha/CCTA antibody (C-Term) (ABIN331511)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivity: Mouse, Rat, Cow, Dog, Rabbit, Hamster, Sheep

Host: Rat

Clonality: Monoclonal

Conjugate: This TCP1 alpha/CCTA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 23c

Product Details anti-TCP1 alpha/CCTA Antibody

Binding Specificity: C-Term

Specificity: Detects a 60 kD protein, corresponding to the apparent molecular mass of TCP-1 alpha on SDS-PAGE immunoblots, in samples from mouse, rat, bovine, sheep, dog, rabbit and hamster origins. This antibody recognizes the C-terminus of mouse TCP-1 (LDDCOOH) but not TCP-1 of primates (LNDCOOH). The COOH group is probably recognized since addition of an alanine (LDDACOOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences. This antibody recognizes other proteins, most notably the p102B' COP subunit of Golgi coatomer. CTA-123 binds protein A and is useful for native precipitations of coatomer and CTT. This antibody does not react with human Hsp60 protein. This antibody also detects a molecular mass band of approximately 92 kD on immunoblots.

Purification: Protein G purified

Immunogen: A purified recombinant mouse TCP-1a construct encoding the C-terminal half of the 1.8 kb full-length Tcp1b gene expressed in E. coli.

Isotype: IgG2c

Application Details

Application Notes: Approved: ICC, IHC, IP, WB (1:1000)

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Alternative Name: TCP1

Background: Name/Gene ID: TCP1

Synonyms: TCP1, CCTA, CCT-alpha, D6S230E, T-complex 1, TCP-1-alpha, Tailless complex polypeptide 1, CCT1

Gene ID: 6950