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TCP1 alpha/CCTA antibody (C-Term)

This anti-TCP1 alpha/CCTA antibody is a Rat Monoclonal antibody detecting TCP1 alpha/CCTA in WB, FACS and IP. Suitable for Human, Mouse, Rat, Cow, Dog, Hamster, Monkey, Guinea Pig, Pig, Drosophila melanogaster, C. elegans and Plant.
Catalog No. ABIN331513

Quick Overview for TCP1 alpha/CCTA antibody (C-Term) (ABIN331513)

Target

See all TCP1 alpha/CCTA (TCP1) Antibodies
TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Reactivity

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Human, Mouse, Rat, Cow, Dog, Hamster, Monkey, Guinea Pig, Pig, Drosophila melanogaster, C. elegans, Plant

Host

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Rat

Clonality

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Monoclonal

Conjugate

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This TCP1 alpha/CCTA antibody is un-conjugated

Application

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Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone

91a
  • Binding Specificity

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    C-Term

    Specificity

    Recognizes an epitope in the C- terminus of mouse TCP-1a (465-AKLRA) and will detect an ~60 kD protein, corresponding to the apparent molecular mass of TCP1 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, pig, and rabbit origins, as well as Saccharomyces cerevisiae (weakly, consistent with the epitope sequence being AKLRS), Drosophila, Schizosaccharomyces pombe and C. elegans. In C. elegans CTA-191 reacts with TCP-1a and another CCT subunit (2). In plants, CTA-191 recognizes TCP -1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP-1a over the region of the epitope AKLRA. It has also been shown that CTA-191 reacts with a subunit of a specialized chaperonin which folds phytochrome (3). Cross reactivity with human Hsp60 has been observed with this antibody in immunoblot analysis.

    Purification

    Protein G purified

    Immunogen

    A purified recombinant mouse TCP-1 construct encoding the C-terminal half of the 1.8 kb full-length Tcp1b gene expressed in E. coli.

    Isotype

    IgG2a
  • Application Notes

    Approved: Flo (1:100), IP, WB (1:1000)

    Not recommended for: IHC-P

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 0.1 mM PMSF, in 50 % glycerol

    Preservative

    PMSF

    Storage

    -20 °C

    Storage Comment

    Store at -20°C.
  • Target

    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

    Alternative Name

    TCP1

    Background

    Name/Gene ID: TCP1

    Synonyms: TCP1, CCTA, CCT-alpha, D6S230E, T-complex 1, TCP-1-alpha, Tailless complex polypeptide 1, CCT1

    Gene ID

    6950
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