PKC alpha antibody

Catalog No. ABIN331530

The Mouse Monoclonal anti-PKC alpha antibody (Clone MC5) (ABIN331530) specifically detects PKC alpha in WB, IHC, IP and FACS. The antibody is reactive with Human, Mouse, Rat, Cow, Dog and Fish samples.

Quick Overview for PKC alpha antibody (ABIN331530)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Human, Mouse, Rat, Cow, Dog, Fish

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: MC5

Product Details anti-PKC alpha Antibody

Specificity: Detects an ~80kD protein, corresponding to the apparent molecular mass of PKC alpha on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, fish (rainbow trout), guinea pig, monkey, pig, rabbit and sheep origins. The epitope of this antibody is within the variable region V3 of PKC alpha (the site of limited proteolysis on the native enzyme). This antibody is specific for PKC alpha and does not detect the other PKC isoforms.

Purification: Protein G purified

Immunogen: Bovine brain PKCa.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (10 μg/mL), IHC (1:50), IP (12.5 μg/mL), WB (1 μg/mL)

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS 0.1 mM PMSF, 50 % glycerol

Preservative: PMSF

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA / PKC-alpha

Background: Name/Gene ID: PRKCA
Subfamily: PKC
Family: Protein Kinase

Synonyms: PRKCA, Aging-associated gene 6, AAG6, Alpha PKC, PKC-A, Pkcalpha, Protein kinase C alpha type, PKC Alpha, PKC-alpha, PKCA, Protein kinase c alpha, Protein kinase C, alpha

Gene ID: 5578

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling