Hsc70 antibody

Catalog No. ABIN334189

This Mouse Monoclonal antibody specifically detects Hsc70 in WB and IP. It exhibits reactivity toward Rat.

Quick Overview for Hsc70 antibody (ABIN334189)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Hsc70 Antibody

Specificity: This affinity purified antibody Detects a ~78kD protein, corresponding to the apparent molecular mass of Hsc70 plant BiP on SDS-PAGE immunoblots, in samples from apple, asparagus, carrot, corn, lettuce, orange, potato, spinach, tomato, Arabidopsis thaliana leaves, seeds and siliques, woody plant bark tissue and tobacco. Although this antibody can be used for immunoprecipitations under native conditions, it is not recommended for immunoprecipitating BiP from tomato lysates. This antibody does not cross react with E. coli DnaK, mammalian Hsc70, recombinant hamster BiP or spinach cytosolic Hsc70.

Purification: Protein G purified

Immunogen: A preparation of spinach Hsc70 proteins comprised of cytosolic Hsc70, BiP and possibly other members of the Hsc70 family of proteins.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IP (10 μg/mL), WB

Usage: Western Blot (Colorimetric): 1 μg/mL. Western Blot (ECL). Immunoprecipitation: 10 μg/mL.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol.

Preservative: PMSF

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8 / HSC70

Background: Name/Gene ID: HSPA8

Synonyms: HSPA8, Heat shock 70 kDa protein 8, Heat shock 70kd protein 10, Heat shock 70kD protein 8, Heat shock cognate protein 54, HSP71, HSPA10, HSC71, HSP73, LPS-associated protein 1, Heat shock 70  kDa protein 8, NIP71, HSC54, HSC70, LAP1

Gene ID: 3312

UniProt: P11142