Claudin 4 antibody (C-Term)

Catalog No. ABIN334265

The Mouse Monoclonal anti-Claudin 4 antibody has been validated for WB, IHC, IF and IHC (p). It is suitable to detect Claudin 4 in samples from Human, Rat and Dog.

Quick Overview for Claudin 4 antibody (C-Term) (ABIN334265)

Target: Claudin 4 (CLDN4)

Reactivity: Human, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Claudin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Claudin 4 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Recognizes the human ~22kD Claudin 4 protein and does not cross-react with the protein at 55kD. Species cross-reactivity: rat ileum homogenates and canine MDCK cell lysates by Western Blot and Immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by Immunohistochemistry.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin 4.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IF (1 - 3 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Target Details for Claudin 4

Target: Claudin 4 (CLDN4)

Alternative Name: CLDN4 / Claudin 4

Background: Name/Gene ID: CLDN4
Family: Claudin

Synonyms: CLDN4, Claudin-4, CPE-receptor, CPETR, CPETR1, Claudin 4, CPE-R, HCPE-R, WBSCR8, CPER

Gene ID: 1364

UniProt: O14493

Pathways: Hepatitis C