PTH1R antibody (AA 146-169)

Catalog No. ABIN334275

This anti-PTH1R antibody is a Mouse Monoclonal antibody detecting PTH1R in WB, IHC and IHC (p). Suitable for Human, Mouse, Cow, Pig, Dog, Monkey and Horse.

Quick Overview for PTH1R antibody (AA 146-169) (ABIN334275)

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Reactivity: Human, Mouse, Cow, Pig, Dog, Monkey, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTH1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PTH1R Antibody

Binding Specificity: AA 146-169

Brand: IHC-plus™

Specificity: Recognizes human PTH/PTHrP receptor, MW 80kD, and the receptor dimer, Mr 160kD. A faint nonspecific band at 110kD is also detected. This antibody does not detect the PTH2 receptor.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%) Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%) Opossum, Chicken (92%) Xenopus (88%).

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to aa146-169 of human PTH/PTHrP Receptor [RRCDRNGSWELVPGHNRTWANYSE]. Species sequence Homology: Mouse & rat (23/24aa). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Bovine, Dog, Horse, Pig (100%), Mouse, Rat, Hamster, Panda, Bat, Rabbit (96%), Opossum, Chicken (92%), Xenopus (88%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Detects PTH/PTHrP receptor in human kidney, vascular smooth muscle and ovary sections. Receptor was also detected in porcine oocytes. Western Blot: 0.5-2 μg/mL. Detects PTH/PTHrP receptor from RIPA lysates of 3T3-L1 pre-adipose fibroblasts. 3T3-L1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P3109-50. Proteins were visualized using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.07 % sodium azide, 30 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Alternative Name: PTHR / PTHR1

Background: Name/Gene ID: PTH1R
Subfamily: Parathyroid hormone
Family: GPCR

Synonyms: PTH1R, Parathyroid hormone 1 receptor, Parathyroid hormone receptor 1, PFE, Pth-related peptide receptor, PTHR, PTH/PTHr receptor, Pth/pthrp receptor, Parathyroid hormone receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTHR1, PTHrP-R, PTHrP-receptor

Gene ID: 5745

UniProt: Q03431

Pathways: Regulation of Carbohydrate Metabolic Process